There are a number of problems with culture-dependent techniques, including a long growth time, inability to handle polymicrobial samples, and reliance on the ability of the bacteria to grow in the media in use. A molecular approach is much faster than traditional methods, and more effective.
Microbial diversity, which can be analyzed by DNA sequences, plays an important role in ecosystems and disease outbreaks. DNA can be extracted from a variety of microbial cells, including environmental cells, which are not amenable to culturing. DNA-based lab technician analysis of microbial communities has been used for a wide range of applications, including understanding how bacteria and fungi interact, assessing the effects of land management on the environment, identifying sources of foodborne pathogens, studying the influence of different hosts on the diversity of microbes, and characterizing microbial diversity in a variety of habitats, such as soil, water, and human tissues.
This method involves the analysis of 16S rRNA. 16S rRNA is found in all organisms and is a highly conserved ribosomal gene. This is a method used to determine the degree of similarity between two microbial communities. The RISA method, i.e. the analysis of ribosomal intergenic sequences, is used in the analysis of various types of microorganisms.
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